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Centromeres and telomeres are indicated by blue and red arrows, respectively, and axial compaction by a black clamp. h Left: contact probability of individual chromosome arms stratified by length.The blue-rumped parrot (Psittinus cyanurus) is a parrot found in the very southern tip of Myanmar, peninsular Thailand, Malaysia, Borneo, Sumatra and nearby ...Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...A PacBio Sequencing library was constructed using the SMRTbell TM Template Prep Kit 1.0 and by following the PacBio 20 kb library protocol; the library molecules, after adapter ligation and damage repair, were size selected for 15 kb and larger using Blue Pippin instrument (Sage Sciences) by following the manufacturer's instructions. The ...BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.

The Pippin platform has since established itself as a key platform for the construction of high-quality libraries for next-gen sequencing. In 2014 Sage Science released a new electrophoresis platform, the SageELF for whole sample fractionation of DNA or proteins, and the PippinHT , a high-throughput version of the BluePippin.The larger sample wells now allow a maximum load of 10 μg (100% up from standard Blue Pippin cassettes). Also the size of the elution module has been increased as well as the surface area of the filtration membrane, bringing about improved sample recovery and reproducibility. Blue Pippin software requirement: v6.31/6.40 CD31

A Blue Pippin device (Sage Science, Inc., Beverly, MA, USA) was used to size select the SMRT Bell template and enrich large fragments (>10 kb). The size-selected library was quality inspected and quantified on an Agilent Bioanalyzer 12 kb DNA Chip (Agilent Technologies, Santa Clara, CA, USA) and a Qubit fluorimeter (Invitrogen, Carlsbad, CA ...The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using "tight" settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...

The Blue Pippin (Sage Science Inc., Beverly, MA, USA) was used to select sizes of the fragments (> 15 Kb). After a second round of DNA end repair, the SMRT Bell™ templates were purified for final sequencing with the PacBio Sequel system (PacBio, Menlo Park, CA, USA). Ten SMRT cells were used to obtain a total of 62.6 Gb (~120X) of raw ...BluePippin. Blue Pippin. WHAT do you use it for? (Applications). Targeted DNA size selection for Next-Gen Sequencing. Collect DNA fragments 100bp to >50kb or ...Cassettes are disposable and pre-cast with agarose and running buffer. Samples are not optically detected.Blotting kits include all the components required to transfer protein, DNA, or RNA samples onto membranes for staining. The materials are grouped specifically for the method and the tissue specimen being implemented, allowing the entire procedure to be completed more rapidly. Using the ... Blue Lakes. On December 23, Sgt. Walsh got a call from the Mini Golden Inn, the Inn said they had some of Noah's items in lost and found. The items were some clothing, chargers for a phone and laptop and a gun lock. However, Noah's phone, computer and gun were missing, so it's believed that Noah was armed when he disappeared into the ...If you'd like us to Pippin your samples for you (plus perform quantification and fragment analysis), the cost is $75 per sample. CLICK HERE to schedule a reservation on BluePippin! (Note, you will need to register for an iLab account.) Fragment Analyzer traces of 5 different Blue Pippin size selected Illumina libraries.

To anchor the scaffolds to chromosomes, 26.79 Gb of clean reads (65.50×) were obtained via Hi-C library sequencing (Supplemental Table 5).Read pairs were mapped to the draft assembly using BWA (version .7.10-r789) (Li and Durbin, 2009).We determined that 85.28% of the reads were correctly mapped to the genome (Supplemental Table 6), including 22.01% uniquely mapped read pairs.

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In order to measure the increase in subread length, long insert libraries were prepared with fragments larger than 4 kb or 7 kb isolated using the Blue Pippin and a 0.75% Agarose Gel Cassette (BLF7510) and compared to a library without Blue Pippin size selection.Individuals were barcoded with 48 unique barcodes (including negative controls with water as input), and we pooled and size-selected each of these subpools of 48 adapters with a Blue Pippin electrophoresis unit (Sage Science, Beverly, MA) using a 1.5% agarose gel cassette and set target size selection to the “tight 450 bp” setting.The PippinHT system is the top choice for higher-throughput workflows. The PippinHT can process 11 size selections at once on a gel cassette (1 of the 12 lanes must be dedicated to an external size marker). The PippinHT accommodates 2 gel cassettes so up to 22 samples can be run at once, and at a quarter of the run time (1 hr 15 m) when ... Authentic Scottie Pippen Chicago Bulls jerseys are at the official online store of the National Basketball Association. We have the Official Bulls City Edition jerseys from Nike and Fanatics Authentic in all the sizes, colors, and styles you need. Get all the very best Chicago Bulls Scottie Pippen jerseys you will find online at store.nba.com.Following ligation of SMRTbell adapters, sheared gDNA was size selected with a 4-kb cutoff using Blue Pippin electrophoresis (Sage Science) to generate a greater fraction of long reads capable of resolving repeat sequencing in the A. xylosoxidans genome. Sequencing was performed using the PacBio RS II platform.User Guide BUF-7510 Sage Science Part Nos: Sage Science, Inc. Suite 2400 500 Cummings Center Beverly, MA 01915 TM High-PassTM DNA Size Selection BLF-7510 doc# 460046

Blue Pippin nucleic acid size selection; Please see the Getting Started page to learn how to work with our Core. Please also see our Instrument List. We encourage you to consult our extensive FAQs in case of questions. Illumina has posted NGS tutorials in this "Beginner's Guide to NGS". Our Shared Equipment includes:polymyxa DSM36 native genomic DNA (2 μg) was obtained directly from the DSMZ collection and a 15kb SMRT bell library was prepared according to the Pacific Biosciences 10-20 kb protocol including additional separation using a Blue Pippin and sequenced with C4-P6 chemistry on 2 SMRT cells. One library consisted of non-size selected (15 kb) DNA ...The nGBS libraries were then size selected on Blue Pippin, discarding fragments smaller than 300 bp and larger than 500 bp long. Sequencing was carried out on an Illumina NextSeq 500/550 using V2 Chemistry and 300 cycles. Sequences were demultiplexed with Illumina's CASAVA data analysis software and proprietary software developed by LGC Genomics.2.0 BLUE-PIPPIN INSTALLATION REQUIREMENTS 2.1 Operating Conditions The BluePippin system is designed to operate under the following environmental conditions: Pollution Degree 2 Installation category 2 Max. Altitude 2000m Indoor use Ambient temperature 17-25oC Humidity 10-80%, non-condensing 2.2 SafetyBlue Pippin Usage. The Blue Pippin is available for user operation for $12/sample after initial training. Alternatively, samples can be dropped off and ran by GSC staff. Please email us at [email protected] for training or to set up a time to drop off samples to be run on the Blue Pippin.

The Pippin platform is typically used to construct genomic libraries for next-gen sequencing. The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power ...

There are different ways of achieving this. (1) Using the molecular weight estimate of the sequencing library from the Bioanalyzer output, one may utilize the DNA:bead ratio chemistry to narrow down the selected size; and/or (2) Use SageElf or Blue Pippin (Sage Science, Beverly, MA) with the desired size as the selection range.Blue is a primary color, which means you cannot create it by mixing other colors. Blue does mix with other colors to create new colors, including mixing with yellow to make green and mixing with red to make violet.Posted on August 3, 2023 by Alex. Range+T Size Selection Sage's DNA size selection technology has been used to help improve PacBio sequencing, particularly for mammalian and complex genome work since the introduction of the BluePippin (2012). Over the last few years, PacBio®'s Hi-Fi circular consensus sequencing … more ».Following ligation of SMRTbell adapters, sheared gDNA was size selected with a 4-kb cutoff using Blue Pippin electrophoresis (Sage Science) to generate a greater fraction of long reads capable of resolving repeat sequencing in the A. xylosoxidans genome. Sequencing was performed using the PacBio RS II platform.Subsequent to library preparation a blue pippin size selection (Sage Science Inc.) was used to isolate target fragment lengths of long range PCR amplicons. Sequencing was performed on a PacBio-Sequel system for 10 and 20 h for cell-culture derived and BAL samples, respectively.pippin: [noun] a crisp tart apple having usually yellow or greenish-yellow skin strongly flushed with red and used especially for cooking.Data Description Background. Yellowhorn (Xanthoceras sorbifolium) (NCBI: txid99658) is a woody oil species [] that belongs to the Sapindaceae family and the monotypic genus Xanthoceras.As an endemic and economically important species in northern China, it is widely used for soil and water conservation owing to its capacity to survive on arid, saline, and alkaline land and in extreme ...

BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an

The purified sample was size selected using the Blue Pippin with a dye-free, 0.75% agarose cassette and U1 marker (Sage Science, BUF7510, Mulgrave, Victoria, Australia) and the 0.75% DF Marker U1 high-pass 30-40 kb vs3 run protocol, with a BPstart cut-off of 35,000 bp. After size selection, the samples were purified with AMPure PB beads ...

BEVERLY, Mass. — November 11, 2014 — Sage Science, a developer of life science products for improving sample preparation processes in molecular biology applications, today introduced the PippinHT, a high-throughput version of the popular Pippin line of automated DNA size selection instruments for use with next-generation sequencers.A PacBio Sequencing library was constructed using the SMRTbell TM Template Prep Kit 1.0 and by following the PacBio 20 kb library protocol; the library molecules, after adapter ligation and damage repair, were size selected for 15 kb and larger using Blue Pippin instrument (Sage Sciences) by following the manufacturer’s …BLU0001 The BluePippin System includes instrument, monitor, keyboard, and mouse. Dimensions are 7"H X 11"W X 21"D, and weight is 15 lbs. Requires 100-240 VAC.Specialties: At Pipkin's Market, we specialize in locally grown produce, specialty groceries and gardening. The garden center carries annuals, perennials and garden ceramics. Established in 1983. Started as a produce market by Ben & Steve Pipkin. Specialty groceries, organics and garden plants were added gradually through the 90s. Focus on locally grown and produced products has grown markedly ...Although they're called halflings, hobbits are a little more than half the height of your average human. They can range from two to four feet in height, but a typical hobbit is around three and a half feet tall. The notable exceptions to this rule are, of course, Merry and Pippin. MORE: Lord Of The Rings: 20 Most Powerful Beings, RankedThe Blue Pippin is utilized for DNA size selection (up to 50kb) for NGS. Desired target sizes or ranges of sizes are entered in software and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination....BluePippin Operations Manual 460013 Rev H 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Blue Pippin Instrument Pulsed-field automated DNA size selection system The Sage Science product portfolio combines pre-cast agarose cassettes with a high-precision, automated solution for the accurate and targeted size-selection of DNA fragments for library construction in NGS workflows Flexibility

Degraded gDNA samples (blue bars) from 2 different cell lines were processed using each of the 4 Demonstrated Protocols (red and green bars). The 40 kb cutoff protocols yield the best results. Over 60% of the recovered DNA (post-size selection) is greater than 50 kb for all 4 DNA test samples.Define pippin. pippin synonyms, pippin pronunciation, pippin translation, English dictionary definition of pippin. n. 1. Any of several varieties of apple. 2. The seed of a fleshy fruit; a pip. …This video provides an overview of the basic types of DNA size selection protocols on the Pippin systems, and how they are programmed in the software interface.Instagram:https://instagram. wichita state trackcollin roushcm oracle cloud loginzavarelli's pizza shop Air Pippen. $665. Kith x Air Pippen 1 'Friends & Family'. 2017. $1,007. Air Pippen. Shop Air Pippen 1 shoes on GOAT. Featuring new, upcoming and iconic styles including the Air Pippen 'Black Varsity Red' 2008, Air Pippen, Air Pippen 'Black Varsity Red' 2008 and more. Buyer protection guaranteed on all purchases. christopher woodwardseats for service members They are all embellished with the same fanciful Early Bird pattern, consisting of a light blue background, adorned with a bird and exotic flowers. The porcelain ...The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ... kansas university bowl game A PacBio Sequencing library was constructed using the SMRTbell TM Template Prep Kit 1.0 and by following the PacBio 20 kb library protocol; the library molecules, after adapter ligation and damage repair, were size selected for 15 kb and larger using Blue Pippin instrument (Sage Sciences) by following the manufacturer's instructions. The ...Follow the links below to our Youtube page for information or training on Pippin Products: General Sage Science Product Overview, 2020 17:42 SageHLS HMW Library System User Guide. These videos are intended to help users visualize some instructions from the user manual: SageHLS YouTube playlist. Individual videos: HLS-CATCH BRCA1 Demonstration 31:40